#Glucose istat high portable#
The blood gas, electrolyte, and biochemistry results were obtained using an iSTAT Portable Clinical Analyzer (Heska Corporation, Fort Collins, Colorado, USA) with CG8 + and CG4 + cartridges. Some subsamples were used for making blood films on clean glass microscope slides, some were stored on ice in sterile plastic vials for future analyses, and others were loaded into the CG-8 + and CG-4 + iSTAT cartridges within 10 minutes of sample collection. The blood was then immediately divided into sub-samples. All turtles were manually restrained and blood samples of approximately 2.5 mLs were obtained from either the left or right dorsal jugular sinus using a heparinized 22 gauge needle attached to a 3.0 mL syringe. These images can be used to identify individuals in the future. In addition to obtaining a blood sample from each turtle, photographs were taken of each side of the head, as well as the carapace. The line was clearly visible underwater and remained on for at least a day, but disappeared soon after (Hirschfeld, personal observation). To avoid capturing the same individual more than once, a line of white zinc oxide ointment was applied to each turtle’s carapace after a blood sample had been obtained and before the animal was released. Both study sites are shallow bays of similar size, sheltered from wave exposure, and were identified as important foraging and resting areas where individual turtles show very high site fidelity. Fourteen turtles were captured, examined, and sampled from La Loberia Beach (0 u 55 9 40 0 S, 89 u 36 9 43 0 W) on 30 June, 2013 and 14 turtles were similarly captured and sampled on 1 July, 2013 at a second site at Punta Carola (0 u 53 9 26 0 S, 89 u 34 9 46 0 W). Blood samples were obtained within approximately 5 minutes of capture. A few turtles were sampled while still partially in water when a receding tide exposed rocks, which prevented turtles from being carried safely onto the sandy beach. Turtles were aligned with their heads facing the water so that the slight incline of the beach assisted efforts to obtain blood samples from the dorsal jugular vein. The animals were carried to shore and placed on the beach. were captured in shallow water within 100 m from the shore by swimmers using standard snorkel, mask, and fins.
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